The Importance of Highly Valued Traits Essay Example for Free

The Importance of Highly Valued Traits Essay Ancient Anglo-Saxon society was based around four highly valued traits; Courage, Generosity, Loyalty, and Courtesy. It is of these four characteristics in which many epic tales were created, and thus passed down for many years through oral account. All of these attributes compile to form what we consider today as a heroic figure. The poem Beowulf denotes all of these traits through many of the heroic characters throughout the course of the poem. The attribute of courage is clearly evident throughout the entire poem. Everything Beowulf does is a direct link to courage. Beowulf is a perfect example of an Anglo-Saxon point of view on how much more important courage and honor are as compared to failure. In this quote, it is shown how determined he is to kill Grendel and gain valor. Of your people or die in battle, pressed/ In Grendels fierce grip. Let me live in greatness/ And courage, or here in this hall welcome/ My death! (632-638). Another prime example of Beowulfs courage is demonstrated shortly before he murders Grendel when he says, Grendel is no braver, no stronger/Than I am! I could kill him with my sword (677- 678). Grendel has killed countless soldiers in Herot for years, but Beowulf claims that not only can he kill him, but also he can do it without any weapons or armor. By doing this, Beowulf takes what seems like a very large risk in a battle and sacrifices it for glory. During the Anglo-Saxon Era, being a hero was very important, but being a hero who accomplishes both the notion of courageous fighting against an evil monster, and doing it with style makes him even more so. Shortly after murdering Grendel, Beowulf yet again demonstrates his courage when he makes the statement Against this monster: when he comes to me/I mean to stand, not run from his shooting (2524-2525). By saying this, it further promotes his right to be the epic hero. Another important characteristic in the poem is Loyalty. A prime example of Loyalty in the poem could be the Golden Torque that is given to Beowulf. The necklace that Wealhtheow gives Beowulf is a symbol of the relationship of loyalty between her people and Beowulf. Because tokens of appreciation and  the like were very vital in Anglo-Saxon time periods, this concept of this keepsake is essential when it is given to Beowulf. An example of Loyalty shown for Beowulf is another key factor in the poem. All of Beowulfs/Band jumped from their beds/Determined/To protect their prince if they could(794-797). This quote is showing how useless Beowulfs men are to him, but at the same time showing their loyalty. In the quote, the word if is used to describe how they would protect their price if they could. Although they are probably unable to do so, it is the loyalty that is imperative. During the Anglo-Saxon Era, the fact of loyalty to ones king or leader is more important than ones ability h imself. As long as a man was loyal to his leader and was willing to try his best and give his life up for him, that is greatest thing a man could give. Courtesy is another example of a necessary trait in the heart of an Anglo-Saxon hero. A hero could have all other traits and not be respected if he is not courteous to his men and to his foe. One example of when Beowulf is very courteous to his foe, is when he first goes to fight Grendel, and Beowulf takes off his armor and puts down his weapon. Not only does this make him a very noble foe, but also helps his ego. Not even the sharpest of swords could have cut/ It through, broken it off the monsters/ Arm and ended its life, as Beowulf/ Had done armed only with his bare hands (987-990). Lastly, one trait that should never be overlooked is Generosity. Although Beowulfs crossing of the sea to help Hrothgar could very easily be considered a generous act in its own, another character in the poem comes to mind when generosity is considered. Welthow, Beowulfs wife, is very generous when all the warriors sit down to a feast in the Mead hall before the battle between Beowulf and Grendel. Then Welthow went from warrior to warrior,/Pouring a portion from the jeweled cup/For each (620-622). Welthow, wanting nothing except for her husband to succeed in defeating this beast seems as if she is doing all she can in order to help. In order for someone to be considered a heroic figure in Anglo-Saxon times, one had to have four main qualities; Loyalty, Courage, Generosity, and  Courtesy. In the epic poem Beowulf, Beowulf illustrates that he is an ideal paradigm for the expression of an epic hero. By being courageous, loyal, generous, and courteous, Beowulf lays the ground not only for the future king of his people, Wiglaf, but also to many epic heroes to come.

Nutrition And Childrens Learning

Nutrition And Childrens Learning Abstract Does what our children eat affect their learning attainment and achievement? And if yes how serious is the effect? Those serious questions have been matters of concern among parents, educators, and health professionals. Prior studies provided evidences on the strong relationship between nutrition and education progress. This research paper is intended to highlight and consolidate the various factors that implicate learning. Also, it illustrates the negative effects of imbalanced diets on childrens health and academic future. This research suggests the serious relationship between prenatal mothers eating habits, breast feeding, anemia, mineral deficiencies, socioeconomic status, malnutrition and obesity from one side and the childrens learning outcomes in the other side. Numerous serious educational issues are addressed in this paper. Including, but not limited to, attention span problems, information processing impairment, information retention issues, cognitive growth complications, and working memory impairment conditions. Eating habits affect equally the childrens physical and mental growth. Therefore, introducing balanced meals to children, especially breakfast, is recommended to ensure healthy growth. Introduction Cognitive development, represented in thinking and learning abilities, is implicated notably by the interaction between nutrition, hereditary genes and the surrounding environment. All these components/factors combined implicate the cognitive growth of children. The role of nutrition in children physical and mental development starts during the early years of their life. Many studies have focused on the relationship between nutrition and childrens mental and physical health. Behavior also is implicated by the quality and quantity of nutrients the child receives. The role of health and nutrition in promoting child development and educational outcomes is increasingly being recognized(Bundy, 1997; Bundy Guyatt, 1996). Research findings tie the mothers nutrition during pregnancy to the childrens brain health and development after birth and during the first two years of life. The childrens cognitive development starts before birth. Furthermore, some researchers suggested that breast feeding augments healthy cognitive development and higher IQ levels. Breakfast is the most important meal especially for preschoolers. Any nutritional disorder, especially during the early stages of life, can lead to serious mental and cognitive issues. Malnutrition and obesity, both, disturb the mental and physical development of children equally. Proper nutrition has always been linked to progressive cognitive development. Malnutrition can results from administrating unbalanced meals or can be secondary to certain medical conditions. Malnutrition leads to deficiency in some essential minerals and other elements. Iron and zinc depots are always depleted by malnutrition. Therefore, malnourished children are always susceptible to recurrent infections which impede their school attendance and thus their learning progress. Moreover, it affects the childrens behavior and social interaction with others. If under-nutrition was evaluated as a negative health risk, overfeeding is viewed as health jeopardy as well. Overfeeding leads to obesity which interferes with the childrens various aspects of growth. Parents should promote healthy eating habits among their children to enhance their mental as well physical growth. Literature review The core of literature on child nutrition and its projecting implications on school performance are addressed in five major areas: (1) nutrition during pregnancy and its prenatal effects on fetuses (2) socioeconomic and medically-induced food insufficiency and malnutrition, (3) essential elements deficiency such as Iron and zinc, (4) effect of eating breakfast, and (5) obesity and its health and educational implications. Extensive research work suggested that chronic hunger, iron deficiency, and poor breakfast patterns are frequently interrelated (Taras, 2005, p 199). Pregnancy is a crucial phase of development. Improper nutrition during pregnancy affects the newborns more than their mothers. Imbalanced supply of certain nutrients during pregnancy leads to serious fetal developmental or congenital malformations. For instance insufficient dietary folic acid or B9 during pregnancy results in motor disabilities and cognitive impairment in offspring. Vitamin B9 is essential in the proper functioning of brain as well central and peripheral nervous systems. Lower levels of B9 have been connected to chronic depression and other mental disorders, as well as in birth defects that affect the neural tube cells (NutritionalSupplementsCenter.com, 2005). Those fore-mentioned serious health defects are found to forgo schooling and engaged in activities besides learning at schools. Moreover, interrupted sleep is one of symptoms of folic acid deficiency. Interrupted sleep is one of the major issues that negatively implicate childrens learning. Interrupted sleep lowers their attention, retention and concentration levels. Therefore, it can be concluded that lower levels of Folic acid insult the children learning process directly. Whether in the form of interrupted sleep issues or manifest itself as mental retardation and/or emotional instability. In the postnatal life, it was suggested that breast feeding is interrelated to IQ levels. Studies in this regard involved more than 3,000 breast-fed infants in New Zealand and England. This study revealed that breast feeding is responsible for increasing the average IQ with around 7 points. However, the same studies indicated that this IQ spurt can only take place under certain hereditary conditions. Breast-fed infants who carry special gene called FADS2 have great chances to have higher IQ than their counterparts who dont have it. Studies suggest that IQ scores are somehow correlated to school performance. Students with above average IQ typically do well in school especially in achievement tests. However, their scores might not be conclusively attributed to their intelligence. Certainly, intelligence does play a significant part in school achievement, but many other factors are also involved. Motivation, quality of education and family socioeconomic status; are all among the other factor involved in elevating the IQ. Conversely, some research studies pointed that for some reason some students dont perform well in the classroom although they have high IQ scores. Meanwhile, other students achieve at higher levels than it would anticipated from their IQ scores alone (J. E. Davidson, 2003; p. 155-156). Balanced healthy meals in the early childhood, especially in the first two years of life, are required to meet their accelerated mental and physical growth development. Therefore, it is their adult partner responsibility to provide them with a variety of nutritional diet array after the age of two. Nutritional meals should include whole grain, dairy products, protein, vegetables and fruit. Excessive intake of carbohydrates, salt and saturated fat should be avoided as possible. Whole grain is an important source of minerals and fibers. Mineral and fibers are essential for healthy growth and development. Healthy balanced diets help developing healthy strong children both physically and behaviorally. Nutritionally rich diets notably improve the childrens attitude. As children grow their share of healthy nutrition should proportionally increase to meet the growth spurt they experience. Their diet should include all the elements that support their physical growth. That includes plenty of Calcium for stronger bones and teeth as well vitamin C. raising healthy children in the early childhood guarantees successful learners in the adulthood. Sound body sound mind has proven to be true. Let alone balanced healthy meals help children establish positive relationships with their counterparts and peers; it affects their readiness to school. Poor nutrition may have knock-on effects for their educational achievement and attainment (Jukes, 2006. P14). Negative effects of malnutrition, for the most part, on brain development are manifested as cognitive impairments and/or emotional issues that last throughout their education time. Physically febrile students are subjected, more than others, to frequent sickness attacks. Therefore, they dont attend school regularly and miss more school days than average. Consequently, they miss, in total, long periods of learning. Socioeconomic status as well the general health condition can lead to malnutrition. Regarding the socioeconomic status, information provided by research about the extent of learning disabilities in low-income communities is still not enough. However, research evidence suggests that a significant number of children are affected (Jukes, 2006. P16). For example, studies have found prevalence of serious mental retardation ranging from 5 children per 1000 in Bangladesh, to 17 per 1,000 in Jamaica, 19 per 1,000 in Pakistan (Durkin, 2002). Worth noting, mental retardation is one of the serious developmental cognitive disorders that affect the early childhood. Children with mental retardation have lower to slim chances to enroll in the regular educational system. What seriously hinders their enrollment is their deteriorated mental health condition and/or the parents schooling decision. Socioeconomic status is not the only contributor to malnutrition. Some medical issues impair the absorption of certain essential elements such as Iron and Zinc. Whether Iron and/or zinc deficiencies were medically-induced or due to other etiologies, they lead to major chronic and sometimes fatal complications. Anemia is one of the most commonly wide spread malnutrition disorders. It affects mostly children and young adults. Studies on anemic children indicated that anemic children showed delayed responses than non-anemic Iron-replete children. The research findings reveled that children with iron deficiency respond after repeating orders or stimuli at least twice. However, there was no difference in the IQ level between anemic and non-anemic children. Iron deficiency anemic children were neither able to categorize information nor able to solve problems quickly. They displayed delay in their ability to solve problems than their iron-replete counterparts. All these complications were found to be attributed to iron deficiency. Iron constitutes major element in the CNS composition. Moreover, studies revealed that anemic children suffer from impaired vision. Impaired vision affects the learning process of visual learners. When iron was administrated into the anemic childrens regular meals they showed improvement in their academic performance, attitude and general health conditions. Zinc is an example of another essential element needed for children and adolescents healthy growth. Zinc is very essential for working memory. Research work disclosed that malnourished children suffer from severe information retention issues. Furthermore, they showed inability to focus their attention normally in any given task. Accordingly, they experienced severe learning impairment and performed poorly at school. Researchers concluded that zinc-related learning impairment was mainly due to their inferior attention span than normal children. Breakfast is not only the most important meal that feeds body with most energy but also it contributes to most nutrients adequacy. Breakfast consumers are more likely to have better overall diet quality and micronutrient and macronutrient and fiber intakes that more often align with current dietary recommendations (US dept. of Health, 2005). Studies revealed that breakfast skippers are more susceptible to obesity. Moreover, they are less likely to be engaged in any physical activities. Several studies support the benefits of breakfast intake. Research findings revealed that constant intake of breakfast meal enhances the childs cognitive functions; academic performance; academic achievement; school attendance rates; psychosocial condition; and mood. Breakfast consumption could impact cognitive performance by alleviating hunger the prevalence of which is well documented and has been associated with emotional, behavioral, and academic problems in children and adolescents (Rampersaud, G. ; Pereira M.; Girard, B.; Adams, j.; Metzl, J.; 2005). Studies revealed that socioeconomic status as well breakfast composition, are among the factors that interfere or augment the magnitude of academic performance. There is extensive proof that daily breakfast intake affect greatly the health condition of children and adolescents. Healthy breakfast should include variety of food groups. Studies indicated that breakfast skippers show low performance compare to others. Decreased attention and other memory related issues were also reported among breakfast skippers. Attention and retention impairment were also reported. Weight gain and its physiological and academic complications are among the health hazards associated with skipping breakfast. Overweight is another crucial factor which impacts the learning process equally to malnutrition. Researchers have studied the relative relationship between birth weight and weight gain during the first two years of life for learning outcomes. The study findings suggested strong relationship between learning expectations and weight gain. Abnormal weight gain increases the risk of failing at least one grade in school. Moreover, further studies indicated that child oversize causes impaired cognitive development and poor academic achievement. Researchers attributed poor academic achievement in obese children to the lack of self-confidence and motivation. Psychological burden associated with obesity is another main factor that implicates the overweight children academia. Conclusion Childrens performance in school is impacted by many etiological factors. Children are expected to perform well in school until some interfering factors disturb their progress. Prenatal nutrition can affect the childs adult life either positively or negatively. Improper nutrition during pregnancy can lead to both serious developmental and congenital defects. These defects are responsible for cognitive and physical growth delay or cessation in severe cases. Children with such defects are expected to perform very poorly in school. In the same time, they are not expected to achieve what they suppose to attain in their corresponding age time frame. Breast feeding was suggested to positively alter the childrens IQ levels. However, studies showed that it does affect it only if the favorable hereditary variable was present. FADS2 is the genetic variable that helps absorb fat in the mothers milk and provide the brain with all the fat needed for healthier growth. Thus, breastfeeding does elevate the childrens IQ by 7 points only in the presence of FADS2 gene. Socioeconomic status interferes with providing healthy comprehensive meals. Therefore, children from low socioeconomic classes are more susceptible to malnutrition disorders. Malnutrition and imbalanced meals can lead to behavioral disorders, memory impairment, delayed cognitive response and emotional disorders. Anemia can also develop as a secondary complication to certain health/medical issues. Whether Anemia was due to malnourishment or secondary to medical issues, it depreciates the academic performance and impairs the childrens progress in school. Moreover, it interferes with their ability to comprehend, process, and retain any introduced knowledge. Iron and zinc are major elements for healthy brain growth and properly functioning working memory. Children with zinc and iron deficiencies showed remarkably delayed responses to any given instruction, behavioral abnormalities and disturbed working memory. Furthermore, they suffered from sleeping disorders which aggravated their academic status. Visual disorders were reported among anemic children. Visual disorders negatively impact visual learners. Breakfast intake is the most important meal of the day, especially for children. Breakfast is important for the childrens daily performance and behavior at school. Studies revealed that breakfast skippers are more susceptible to obesity when they grow up. Skipping breakfast alters the glucose supply to the brain and put it into the energy saving mode. Consequently, this conservative mode disturbs the memory and the ability to stay focused. Overweight is another burden that traumatizes the childrens academic achievement. Due to the psychological inferiority which obese people experience, they suffer from lack of self-confidence. Low self-esteem, lack of motivation and inability to participate in various activities create unfavorable learning conditions. These conditions, by turn, hinder their academic progress and schooling outcomes. Abnormal weight gain increases the risk of failing at least one grade in school.

High Performance Liquid Chromatography Experiment

High Performance Liquid Chromatography Experiment INTRODUCTION Pharmaceutical Analysis may be defined as the application of analytical procedures used to determine the purity, safety and quality of drugs and chemicals. The term Pharmaceutical analysis is otherwise called quantitative pharmaceutical chemistry. Pharmaceutical analysis includes both qualitative and quantitative analysis of drugs and pharmaceutical substances starts from bulk drugs to the finished dosage forms. In the modern practice of medicine, the analytical methods are used in the analysis of chemical constituents found in human body whose altered concentrations during disease states serve as diagnostic aids and also used to analyze the medical agents and their metabolites found in biological system. Qualitative inorganic analysis seeks to establish the presence of given element or inorganic compound in a sample. Qualitative organic analysis seeks to establish the presence of a given functional group or organic compound in a sample. Quantitative Quantitative analysis seeks to establish the amount of a given element or compound in a sample. The term quality as applied to a drug product has been defined as the sum of all factors, which contribute directly or indirectly to the safety, effectiveness and reliability of the product. These properties are built into drug products through research and during process by procedures collectively referred to as Quality control. Quality control guarantees with in reasonable limits that a drug products Is free of impurities. Is physically and chemically stable Contains the amount of active ingredients as stated on the label and Provides optimal release of active ingredients when the product is administered. Most modern analytical chemistry is categorized by two different approaches such as analytical targets or analytical methods. INTRODUCTION FOR CHROMATOGRAPHY: High performance liquid chromatography is the process, which seperates mixture containing two or more components under high pressure. In this the stationary phase is packed in column one end of which is attached to a source of pressurized liquid mobile phase. High performance liquid chromatography is the fasted growing analytical technique for the analysis of drug. Its simplicity, high specificity and wide range of sensitivity makes its ideal for the analysis of many drugs in both dosage form and biologic fluids. HPLC is also known as High performance liquid chromatography. It is essential form column chromatography in which the stationary phase is consists of a small particles (3-5o µm) packing contained in a column with a small bore (2-5mm), one end of which is attached to source of pressurized liquid eluent(mobile phase). Different Types of Principles: According to the phases involved, HPLC can be classified into several types, which are as follows: Normal Phase Chromatography (NPC) Reverse Phase Chromatography (RPC) Liquid Solid Chromatography or adsorption HPLC Liquid Liquid Chromatography or Partition HPLC Ion exchange Chromatography or Ion exchange HPLC Size exclusion or gel permeation or steric exclusion HPLC 1. Normal Phase Chromatography (NPC): In normal phase chromatography, the stationary phase is more polar then the mobile phase, and the mobile phase is a mixture of organic solvents with out added water (e.g. isopropane with hexane) and the column packing is either an inorganic adsorbent (silica) are a polar bonded phase (cyanno, diol, amino) on a silica support. Sample retention in normal phase chromatography increases with the polarity of mobile phase decreases. They are eluted in the order of increasing polarities. 2. Reverse Phase Chromatography (RPC): In reverse-phase chromatography, the stationary phase is less polar than the mobile phase and the mobile phase is a mixture of organic and aqueous phase. Reverse-phase chromatography is typically more convenient and rugged than the other forms of liquid chromatography and is more likely to result in a satisfactory final separation. High performance RPC columns are efficient, stable and reproducible. In this, the solutes are eluted in the order of their decreasing polarities. These are prepared by treating the surface silanol group of site with an organic chloro silane reagent. INSTRUMENTATION: RECORDER SCHEMATIC DIAGRAM OF HPLC a. Pumps: Pumps are required to deliver a constant flow of mobile phase at pressures ranging from 1 550 bar pumps capable of pressure up to 6000 psi provide a wide range of flow rates of mobile phase, typically from 0.01-10ml min-1. Low flow rates (10-100à ¯Ã‚ Ã‚ ­l min-1) are used with micro bore columns, intermediate flow rates (0.5-2ml min-1) are used with conventional analytical HPLC columns, and fast flow rates are used for preparative or semi preparative columns and for slurry packing techniques. Mechanical pumps of the reciprocating piston type view a pulsating supply of mobile phase. A damping device is there fore required to smooth out the pulses so that excessive noise at high levels of sensitivity or low pressure does not detract from detection of small quantities of sample. This type of pump is mostly used. Dual piston reciprocating pumps produce an almost pulse free flow because the two pistons are carefully faced so that as one is filling the other is pumping. These pumps are more expensive than single piston pumps but are of benefit when using a flow sensitive detector such as ultraviolet or refractive index detector. b. Injection Systems: Injection ports are of two basic types, (A) those in which the sample with injected directly into the column and (B) those in which the sample is deposited before the column inlet and then swept by a valving action into the column by the mobile phase. c. Columns: HPLC columns are made of high quality stainless steel, polish internally to a mirror finish. Standard analytical columns are 4-5 mm internal diameter and 10-30 cm in length. Shorted columns (3-6 cm) containing a smaller particles size packing material (3 or 5 à ¯Ã‚ Ã‚ ­m) produce similar or better efficiencies, in terms of the number of theoretical plates (about 7000), that those of 20 cm columns containing 10 à ¯Ã‚ Ã‚ ­m irregular particles and are used an short analysis time and highest throughput of samples are required. Micro bore columns of 1-2 mm internal diameter and 10-25 cm in length have certain advantages of lower detection limits and lower consumption of solvent, the latter being important if expensive HPLC grade solvents are used. HPLC are also being carried out on the semi preparative scales by using columns of 7-10 mm or 20-40 mm internal diameter respectively. d. Detectors: The most widely used detectors for liquid chromatography are Detector Analytes Solvent Requirements Comments UV-Visible Any with chromophores UV-grade non UV absorbing solvents Has degree of selectivity and useful for many HPLC applications Fluorescence Fluorescent compounds UV-grade non UV absorbing solvents Highly selective and sensitive, often used to analyze derivitized compounds Refractive index Compounds with different RI than mobile phase Cannot run mobile phase gradients Limited sensitivity Conductivity Charged or polar compounds Mobile phase must be conducting Excellent for ion exchange compounds Electrochemical Readily oxidized or reduced compounds, specially biological samples Mobile phase must be conducting Very selective and sensitive Mass-Spectrometer Broad range compounds Must use volatile solvents or volatile buffers Highly sensitive. Many modes available. Needs trained person Theoretical principles of HPLC: a. Retention time: The time is required between the injection point and the peak maximum is called the retention time. It is denoted as the Rt. It is mainly useful for the qualitative analysis for the identification of compound. b. Capacity factor: It represents the molar ratio of the compound in the stationary phase and the mobile phase. It is independent of column length and mobile phase flow rate. It is denoted as the k. It should be kept 1-10. If k values are too low it is likely that the solutes may be adequately resolved and for high k values the analysis time is too long. It can be calculated by tr t0 k = - t0 tr = Retention time, t0 = Dead time. c. Tailing factor: Closer study of a chromatographic show that the Gaussian forms is usually not completely symmetrical. The graph spread out to a greater or lesser extent, forming a tail. It reduces the column plate number which intern influences the resolution. Tailing is mainly due to deteriorated column, overloading column, extra column-volumes, and incompatibility of sample with standard and/or mobile phase. Practically it can be calculated or determined at 10% of the total peak height. It must not be greater than 2.0 d. Resolution: The degree of separation of one component from another is described by the resolution. It is generally denoted by Rs. It is measured as the difference in retention time and the arithmetic mean of the two peak widths. tr2 tr1 Rs = 0.5(w1 + w2) tr2 = Retention time of first peak w1 = width of first peak tr1 = Retention time of second peak w2 = width of second peak e. Theoretical plates: It is important property of the column. It reflects its quality of separation and its ability to produce sharp, narrow peak and achieving good resolution of peak. N denotes it. 3500 X L (cm) Theoretical plates = - dp( µm) L = length of the column in cm, dp = diameter of the particle ( µm) It follows that if the exchange is fast and efficient, the theoretical plate will be small in size and there will be large number of plates in the column. f. Height equivalent to theoretical plate (HETP): Number of plates directly proportional to the column length (L) and inversely proportional to the diameter of the particles (dp). The value of H is a criterion for the quality of a column. Lower the HETP, higher is the efficiency of the column. Its value depends upon particle size, flow rate, viscosity of mobile phase. H = L/N L = Length of column, N = No. of theoretical plate HPLC method development: The wide variety of equipment, columns, eluent and operational parameters involved makes high performance liquid chromatography (HPLC) method development seem complex. The main objective of method development is to obtain a good separation with minimum time and effort. Based on the goal of separation, the method development is preceded. The steps involved are: Information on sample, define separation goals Need for special HPLC procedure, sample pretreatment, etc. Choose detector and detector settings Choose LC method, preliminary run; Estimate best separation conditions Optimize separation conditions Check for problems or requirement for special procedure Validation for release to routine laboratory The following must be considered when developing an HPLC method: Keep it simple Try the most common columns and stationary phases first Thoroughly investigate binary mobile phases before going on to ternary Think of the factors that are likely to be significant in achieving the desired resolution. Mobile phase composition, for example, is the most powerful way of optimizing selectivity whereas temperature has a minor effect and would only achieve small selectivity changes. pH will only significantly affect the retention of weak acids and bases. VALIDATION OF ANALYTICAL METHOD IN PHARMACEUTICAL ANALYSIS: Validation is documented evidence, which is completed to ensure that an analytical method is accurate, reproducible and robust over the specific range. The quality of the analytical data is a key factor in the success of a drug development program. The process of method development and validation has a direct impact on the quality of these data. Method validation: Method validation is the process to confirm that analytical procedure employed for a specific test is suitable for its intended use. Method needs to be validated or revalidated Before their introduction into routine use Whenever the conditions changes for which the method has been validated , e.g., instrument with different characteristics Whenever the method is changed, and the change is outside the original scope of the method. Depending on the use of the assay, different parameters will have to be measured during the assay validation. ICH and several regulatory bodies and Pharmacopoeia have published information on the validation of analytical procedures METHOD VALIDATION PARAMETERS: SPECIFICITY. ACCURACY. PRECISION. LINEARITY. ROBUSTNESS. SOLUTION STABILITY. The goal of the validation process is to challenge the method and determine the limit of allowed variability for the conditions needed to run the method. The following statistical parameters are to be determined to validate the developed method. Correlation coefficient(r): When the changes in one variable are associated or followed by changes in the other, it is called correlation. The numerical measure of correlation is called the coefficient of correlation and is defined by the relation. à ¯Ã‚ Ã¢â‚¬Å" (x x) (y -y) r = à ¢Ã‹â€ Ã… ¡ à ¯Ã‚ Ã¢â‚¬Å"(x -x) 2 à ¯Ã‚ Ã¢â‚¬Å"(y -y Regression equation: Regression equation= I + aC Y2 Y1 a = slope = X2 X1 I = Intercept = regression a C As a percentage of mean absorbance. 3. Standard Deviation: S = à ¢Ã‹â€ Ã… ¡ à ¯Ã‚ Ã¢â‚¬Å" (X- X!) 2/N 1 Where, X = observed values X! = Arithmetic mean = à ¯Ã‚ Ã¢â‚¬Å"X/N N = Number of deviations For practical interpretation it is more convenient to express S in terms of percent of the approximate average of the range of analysis is used in the calculation of S. This is called co-efficient of variation (C.V) or percent relative standard deviation (%RSD). C.V OR %RSD = 100* S/ X! Criteria for Validation of the Method CHARACTERISTICS ACCEPTABLE RANGE Specificity No Interference Accuracy Recovery (98-102%) Precision RSD Linearity Correlation Coefficient(r)>0.99 Range 80-120% Stability >24h or >12h DRUG PROFILE RIZATRIPTAN BENZOATE: Structure: Chemical name : N,N diethyl -5-(1H-1,2,4-triazol-1-1-ylmetyl)-1H Indole-3 Ethanamine monobenzoate Molecular Formula : C15H19N5.C6H5COOH Molecular weight : 391.47 Description: White crystalline powder Melting point: 178-1800C Solubility: Sparingly soluble in water and methanol Storage: Air tight container protect from light. Drug Category: Anti migraine drug THERAPEUTIC RATIONAL RIZATRIPTAN BENZOATE: CLINICAL PHARMACOLOGY: Mechanism of action: Rizatriptan binds with high affinity to human 5-HTIB and 5-HTID receptors leading to cranial blood vessel constriction. Pharmacokinetics: Absorption: Completely absorbed from GI tract, absolute bioavailability is 45% plasma peak concentration attained with in 1-1.5 hours (conventional tablet )or 1.6-2.5 hours (orally disintegrating tablet)after oral administration. Distribution: Crosses placenta and is distributed in to milk in animal, no studies in pregnant or nursing women. Metabolism: Metabolized principally via oxidative deamination by Mao-A to an inactive indole acetic acid metabolite Elimination: Excreted principally in urine(14% of dose as unchanged drug and 51 % a indole acetic acid metabolite Adverse effects: Dry mouth Dizziness Pain tightness/pressure in neck/throat/jaw. Nausea Chest pain Parasthesia Fatigue Dosage and administration: The dose range of Rizatriptan benzoate is 10-30mg orally once daily.Rizatriptan benzoate can be administer orally disintegrating tablet with out meals. LITERATURE REVIEW Sasmitha Kumar et al: has been developed UV spectroscopic method for estimation of Rizatriptan benzoate.The drug shows maximum absorption at 277 nm and 281 nm and obeys beer-lamberts law in the concentration of 0.5-20  µg/ml at 277 nm and 0.5-80  µg/ml at 281 nm respectively. The percentage recovery was found to be 97-100%. Madhukar et al; has been developed reverse phase high performance liquid chromatographic method for determination of Rizatriptan benzoate. The proposed method utilized column L1 inertsil ODS-3v, 250 nmx4.6 mm having particle size, 5 µm. The mobile phases were comprised of A, B of Acetonitrile and buffer pH 6.5 at UV detection 225 nm.The method shows recovery 96.64-97.71 Sachin jagthap et al; has been developed stability indicating reversed phase high performance liquid chromatographic method for the determination of Rizatriptan benzoate in bulk powder and in pharmaceutical formulations. The method utilizes c18 column having dimension 250mmx4.6 mm having particle size,5.0  µm using a mobile phase 0.01M sodium dihydrogen phosphate buffer: Methanol , at a flow rate 1ml/min at ambient temperature and detected at 225 nm.and the method was validated according to ICH guidelines Quizi zhang et al: has been developed, a high performance liquid chromatographic method for the determination of Rizatriptan benzoate in human plasma.using asingle step liqid liqid extraction with metyl tertiary butyl ether, the analytes separated usig amobile phase consisting of 0.05%v/v triehylamine in water adjusting ph 2.75 with 85% phosphoric acid and acetonitrile.fluroscence detection was performed at an excitation wavelength of 225 nm and an emission wavelength of 360 nm.The linearity for rizatriptan was within the concentration range of 0.5-50ng/ml. Rajendra Kumar et al: has been developed and validated stability a stability indicating high performance liquid chromatographic method for Rizatriptan benzoate.The force degradation studies were performed on bulk sample of Rizatriptan benzoate. The method utilizes a zorbax SB-CN column with dimension of 250 mmx4.6 mm, 5um column. The mobile phase consists of a mixture of aqueous potassium dihydrogen ortho phosphate (ph3.4), acetonitrile and methanol. Rauza bagh et al: has been developed a spectroscopic method for analysis of Rizatriptan benzoate in bulk and tablet dosage form. The Rizatriptan benzoate shows maximum absorbance at 225 nm. Beers law was obeyed in the concentration range of 1-10 µg/ml. AIM AND PLAN OF WORK The present aim is to develop a new simple and rapid analytical method to estimate the Rizatriptan benzoate The plan of the proposed work includes the following steps: To undertake solubility studies for analytical studies of Rosuvastatin calcium Develop initial chromatographic conditions. Setting up of initial chromatographic conditions for the assay of Rosuvastatin calcium Optimization of initial chromatographic conditions. Validation of the developed HPLC Analytical method according to ICH method validation parameters. EXPERIMENTAL NEW RP-HPLC METHOD FOR THE ESTIMATION OF RIZATRIPTAN BENZOATE IN TABLET DOSAGE FORM A simple reverse phase HPLC methods was developed for the determination of Rizatriptan benzoate in tablet dosage form. Zorbax Eclipse XBD C18 (250 cm ÃÆ'- 4.6 mm) column in isocratic mode with mobile phase Buffer ph 5.0: Methanol (80:20) was used and pH-3 adjusted with tri ethylamine. The flow rate was 1.0 ml/min and UV detection at 225nm. The retention time 3.0 min. The proposed method was also validated. EXPERIMENTAL 1. Instrumentation: Shimadzu LC-10A HPLC Vacuum pump Gelmon science Elico SL-164 double beam UV-Visible spectrophotometer Ultra sonicator 3.5L 100(pci) 2. Chemicals: Water HPLC grade Methanol HPLC grade (Merck) Potassium dihydrogen orthophosphate(AR Grade) Triethylamine (AR Grade) 5.1 OPTIMIZATION: 1. Selection of wavelength: After solubility study for the drug solvent was selected and appropriate concentration of Rizatriptan benzoate standards with solvent were prepared. The solution were then scanned by using doubl beam UV-Visible spectrophotometer the range between 200-400nm.The overlain spectra for the both drug were observed and maximum wavelength was finally selected. 2. Selection of mobile phase: To develop a prà ©cised and robust HPLC method for determination of Rizatriptan benzoate , its standard solution were injected in the HPLC system. After literature survey and solubility data different composition of mobile phase of different flow rates were employed in order to determine the best condition for effective separation of drugs. 3. Selection of column: Initially different C8 and C18 columns were tried for selected composition of mobile phase and quality of peaks were observed for the drugs. Finally the column was fixed upon the satisfactory results of various system suitability parameters such as column efficiency, retention time, tailing factor / peak asymmetry of the peaks. Other parameters such as flow rate, column temperature etc. were selected by varying its value up to certain levels and results were observed. The value at satisfactory results were obtained has been selected for the method. The final selection of chromatographic conditions as follows Optimized chromatographic conditions Preparation of Buffer ph 5.0: Dissove 2.76 gm of potassium dihydrogen orthophosphate in 1000ml of HPLC water plus 5.0 mlof Triethylamine. Mix and adjust PH 5.0 with orthophosporic acid. Filter with 0.45u nylon filter. Preparation of mobile phase: The mobile phase was prepared by mixing Buffer: Methanol (80:20). the solution was then filtered through 0.45ÃŽÂ ¼m membrane filter and sonicated. Preparation of standard stock solution: Standard solution of the pure drug was prepared by dissolving 73.0 mg of Rizatriptan benzoate in 100ml volumetric flask. The drugs were dissolved by using mobile phase as a diluent. Add about 50ml of diluent and sonicate to dissolve. Make up the volume with diluent. Mix well. Further dilute 5.0ml of the above solution to 250ml with diluent, mix well. Preparation of sample solution: Weight and transfer 10 intact tablet in into a100ml volumetric flask. Add about 50ml of diluent and sonicate for 15 min and make up the volume with diluent. Mix well, filter through 25 mm 0.45 u nylon , discard 4ml filtrate. Further dilute 5ml of the solution to 250 ml with diluent and mix well. CONCLUSION The evaluation of obtained values suggests that the proposed HPLC methods provide simple, precise, rapid and robust quantitative analytical method for determination of Rizatriptan benzoate in tablet dosage form. The mobile phase is simple to prepare and economical. After validating proposed method as per ICH guidelines and correlating obtained values with the standard values, satisfactory results were obtained. Hence, the method can be easily and conveniently adopted for routine estimation of Rizatriptan benzoate in tablet dosage form.

Economy :: Unions Labor Work Papers

Unions Why Unions? "Unions are groups of working people who join to talk to employers about wages and conditions of work instead of workers talking to employers on an individual basis."1 Because they speak for everybody, unions can get a better deal for each worker than one employee could by negotiating with the employer. As seen in the short movie "WHY UNIONS?", non-unionized workers talks about the unfair treatment they experience in the work place. Through collective action, workers formed unions so they could have a voice in deciding wages, hours, working conditions and dealing with the many problems arises in the workplace. Unions are not just organizations trying to get more dollars and cents or better working conditions for people who hold union cards. People who don't enjoy the benefits of union protection get benefits too. "The labour movement was in the forefront of the struggles for public health care, for public education, for minimum wages, holidays and employment conditions."2 1 D. Martin, Form War To Peace, Canadian Labour Congress, Quebec, 1991, P.17 2 Notes On Unions, Canadian Labour Congress, Quebec, 1992, P.1 Union Nowadays "Unions are like businesses: their success depends upon attracting and retaining buyers (new members). Recently, one of the major problems faced by the union movement is that few Canadians understand what services unions come into public."3 The public also don't know the union is also helping Canada in other parts of areas such as economy, etc. The public think that the only time unions come into the public's eye is as a result of strike or withdrawal of services. This is because of the impressed strikes held by the union workers. The memorable one would be the strike held by the Toronto Transit Commission workers back in September 1991. The lack of transportation resulted in inconveniences among the public. The Canadian public becomes upset over conflict. The strikes make the public forget about the contributions of unions in other areas. "From a marketing perspective, the union movement has positioned itself like the nuclear industry -- it receives attention only when it is about to blow up, melt or leak."4 Five important needs for union movements are: 3 Alistair Davidson and Ian Mckinnon, "Unions need to study marketing," The Globe and Mail, May 8 1984, Business section, P.8

Linux Versus Windows NT Essay -- essays research papers

Comparing any network operating system is really like comparing an apple to an orange. To judge between the underlying functionality of each operating system geared towards different networking environments is too broad of a subject to come to a reasonable conclusion. However, one could argue about the advantages and disadvantages pertaining to the Unix based Linux operating system and the infamous Windows NT operating system. I can compare and contrast these two operating systems, but I can’t take a strong stand because it all depends on a number of situations including the different networking environments, the applications that are readily available, and most important what the user wants. The goal is to find out which of these two operating systems is the lesser of two evils by examining their features. I have decided not to discuss cost because it goes without saying: Linux wins the price to performance ratio. What is more important to discuss is the initial hardware and software fees, and maintenance and reliability which often go hand in hand. According to Sunworld, the estimated minimal required hardware costs that would go with a Linux machine is $200. In an NT machine, the minimal hardware cost rose up to $1300. This is because NT requires at least a 486 Pentium with 16MB of RAM. Linux can run fine on a 386 computer with only 8MB of RAM. What do the majority of users need a computer for? Mainly word processing and the use of a database program. An NT user doesn’t have to go with Microsoft Office, but a lot of people do, costing a couple hundred dollars. Linux can run an Office Suite program that is very similar to Office for at least half the cost. Even a free version of WordPerfect 8 is available for Linux. A Linux system comes with a range of development tools (C/C++ Compiler, Perl, Tcl/Tk, Python, CORBA, Ada, Pascal, Lisp, REXX, Java, etc., as well as many text editors and integrated development environments), all of which are free. In order to create software under Linux, there is no expensive package to buy. Many NT users complain about system crashes or the dreaded “blue screen of death.'; NT systems face a lot of down times, which creates a problem for mission critical situations. It is very frustrating to be working on something for a couple of hours and see it vanish or crash. Unix based systems have experie... ...n that path. NT, however, does not provide the source code; therefore, limiting the user to only Microsoft’s networking and programming solutions. The creator of Linux, Linus Torvalds, developed this operating system using past versions of Unix strengths, and avoiding the weaknesses and flaws that have been created for more than 30 years. Linus also got help from would be programmers from all over, many being hackers, to create this sophisticated operating system. Having a wide variety of help and feedback supports the integration of a system that can satisfy most users. NT, on the other hand, is developed by their own team, not taking much consideration to what consumers really want to see. Although this report isn’t very cohesive to intervene between each subject, I think it provides grounds to see clearly how well Linux presents itself over NT. Although Linux is still in its primitive stages, the mark it has put in this world is quite remarkable. These early version of Linux provides a stepping stone for later versions to come, and possibly dominate the market when executives and upper level managers alike come to see that Linux is a great industry solution.

Tanning

Welcome to Four Seasons Tanning, the quintessential consultant for today’s salon owner. From the hottest new arrivals in lotions, to the most advanced tanning beds on the market, Four Seasons gives you everything you need to run a successful salon, and for those thinking of opening a salon. Four Seasons Sales and Service is the one source solution. Saving time and stress by putting decades of experience to work to help your salon profit. Four Seasons Sales and Service is a professional indoor tanning distributor with over twenty years of industry experience that was founded in 1983 by twin brothers, Ronnie and Johnny Allen. Ronnie’s wife owned a small beauty salon, and she decided to add tanning as an additional business. At that time, they were one of the few individuals in the area with any experience on tanning products and tanning equipment. The business started when Ronnie and Johnny worked on their own tanning beds and began to repair other salons in the area. Before long, they began carrying tanning products to help their clients achieve maximum tanning results, and teaching them how to properly take care of their skin while tanning. This was the largest and fastest growing part of the business. What began as a business promoting products and services out of the back of their truck rapidly grew into a large-scale warehouse operation that expanded to shipping nationwide. Four Seasons Sales and Service also have proven management in distribution of and consultation on tanning products, parts, services, and marketing advantages, while also staying well equipped with first-class investigative, design, and problem solving skills. The Allen brothers always reinvested in the company, and prided themselves on taking care of the heart of the business; their employees. Currently Four Seasons Sales and Service provide approximately two-hundred full time professional specialists that are friendly and efficient, and are dedicated to partnering with salon owners to help them achieve their very best. Four Seasons is proud to be the only employee owned company in the Indoor Tanning Industry. This means that Four Seasons' specialists and team members have the ability to share in our company's success. Throughout this paper, you will read and learn about a team of incredibly productive and highly motivated individuals that will settle for nothing less than excellence.

Explain How Constructivist Theory Differs from Traditional Ideas About Teaching and Learning

Constructivist Theory is very similar to the traditional ideas about teaching. The teacher’s roles that are similar include specifying learning objectives, preparing learning activities, and designing assessments. The main difference between Constructivist Theory and the traditional roles of a teacher is â€Å"a shift in emphasis away from the teacher merely providing information and toward the teacher promoting the interaction that makes students’ thinking open and visible.The constructivist theory puts the opportunity for knowledge in the students’ hands more than in traditional ideas of teaching. The teacher acts as a guide for imaginative and exploratory thinking. I would use constructivism in my classroom. I will provide high quality representations. I will use examples that include all of the information necessary in order for the students to understand the topic. The first example that popped into my head was to bring in a cake for each group and to cut t he cake into different portions to help with fractions in math.I will also connect content to the real world. I will try my best to relate the content I am teaching to the students. I know for myself, when I can relate to the content that is being taught, it is more interesting to me and I am more likely to do well in that specific content area. I will promote high levels of interaction. Group work will definitely be present in my classroom. Something I learned from this section is that the Constructivist Theory can be used with all grades and with all ages.